Heavy Metal ion chelation property in BN

BN is very unique as it has been proven to detoxify the accumulated lead, cobalt, cooper, iron and platinum in our bodies. We have conducted extensive research on this topic. 

●EFFECTS OF BIONORMALIZER ALONE OR IN COMBINATION WITH ANTICANCER DRUGS ON CANCER CELLS. A PRELIMINARY STUDY (Platinum chelator action)　http://osri.asia/oncology/fm12-effects-of-bionormalizer-alone-or-in-combination-with-anticancer-drugs-on-cancer-cells-a-preliminary-study

●NATURAL ANTIOXIDANTS IN NUTRITION, HEALTH AND DISEASE PREVENTION http://osri.asia/general-medicine/fm8-2

●THE EFFECTS OF BIO-NORMALIZER ADMINISTRATION TO THE RATS WITH LUNG INFLAMMATION INDUCED BY COBALT IONS　http://osri.asia/toxicology/or8

●NATURAL OCCURING ORAL CHELATORS AGAINST LEAD AND COBALT TOXICITY　http://osri.asia/toxicology/fm7

●PROTECTIVE EFFECT OF BIO- NORMALIZER AGAINST ASBESTOS- INDUCED LUNG INJURY. http://osri.asia/toxicology/or7

●Effects of Bio-Normalizer on Oxidative Stress in Iron-Overloaded Rats http://osri.asia/toxicology/pa33

●Suppression by Antioxidative Chelators of Toxic Effects of Iron Overload in Rats　http://osri.asia/toxicology/pa24

●Free Radical Mechanism of Iron Toxicity Effects of Iron Chelators and Antioxidants)　http://osri.asia/toxicology/pa23

●Natural Occurring Antioxidant and Chelators Against Harmful Effects of Transition Metals　http://osri.asia/toxicology/pa22

●Prooxidant Effect of Transition Metals (Iron and Cooper) in Biological Systems　http://osri.asia/toxicology/pa21

●Antioxidant Action of Bio-Normalizer on Asbestos- and Cobalt- Induced Lung Injury　http://osri.asia/toxicology/pa6

This article was written by Miragrosa S. Cuadra, who serves as the Director of the Osato Sano Research Institute.

Miragrosa S. Cuadra: After completing a master's degree in microbiology at the University of the Philippines Diliman, he worked as a graduate student in the International Post-Graduate University microbiology course sponsored by UNESCO and the Japanese government. He conducted research at various universities, including Osaka University, the University of Tokyo, Tohoku University, Kyushu University, and Kyoto University before joining SunO International., Ltd. She assumed the role of an analytical researcher at SunO International.

Heavy metals like lead, arsenic,  antimony, cadmium, chromium, cobalt, copper, iron, manganese, mercury, nickel, platinum, silver, tellurium, tin, uranium, zinc to name a few are commonly found in the environment and diet that many of these metallic elements are found within the body, but mostly in small amounts. It has been found that some of these metals like  iron, manganese, zinc, cobalt, copper, molybdenum, sodium, potassium in the form of metallic salts  have essential functions in the physiological processes, but in traces. It can also be noted that the deficiency of some metals may cause pathological conditions such as iron-deficiency anemia. But, there are also some that exist in the body without any function and exist at levels low enough to be tolerated. However, there are chances of overexposure wherein the concentration of metallic elements rises above the toxic threshold leading to disruption of cellular functions. Excess exposure to metals can be toxic and can contribute to human diseases, including cancer, hepatic diseases, and neurodegenerative diseases (such as Alzheimer’s, Parkinson’s, and Huntington’s diseases). Metals such as mercury and lead that are widely used in modern industry nowadays, do not have any known physiological function in human and are considered potent toxicants.

With the increasing environmental exposures and new  food formulations with various metallic compounds are causing growing concerns that result to imbalances in metal content and excess exposure may contribute to the pathogenesis of various human diseases.

That said, the oxidative stress is a fundamental molecular mechanism underlying metal-induced toxicity. Most metals are redox-active, especially transition metals, such as iron, copper, manganese, and zinc. They can undergo redox cycling reactions resulting in the production of reactive oxygen (ROS). For example, in Fenton’s reaction, iron reacts with hydrogen peroxide to produce hydroxyl free radicals, one of the most reactive ROS molecules. Excess intracellular ROS eventually disrupts the intracellular redox state and energy production resulting in oxidative stress, which manifests itself in the modification of cellular biomolecules, such as DNA, lipids and proteins, the dysfunction of mitochondrial respiration, protein folding, DNA repair processes, inflammation to name a few. These cellular characteristics are frequently observed in the pathogenesis of human diseases have been implicated in the development of aging as well as various ailments including cancer, respiratory, cardiovascular, neurodegenerative, and digestive diseases.

Since, Bio-Normalizer is a health food supplement  known to have antioxidant action, there were studies conducted to elucidate its role in curbing metal intoxication. As follows are some of the Bio-Normalizer researches on metal-associated cellular toxicity, to wit:

1. Antioxidant Action of Bio-Normalizer on Asbestos- and Cobalt-induced Lung Injury

To elucidate the antioxidant action of Bio-Normalizer on asbestos- and cobalt-induced lung inflammation, two groups of Wistar rats were given intratracheal administration of 15mg of  chrysotile asbestos and 2.5 mM of CoSO₄, respectively, combined with 10-50 mg/ml Bio-Normalizer. Bio-Normalizer administration substantially lessen the inflammation and fibrosis of lung tissue by suppressing free radical-mediated lung injury caused by lipid peroxidation and over production of ROS by pulmonary phagocytes post-induction with asbestos and cobalt. Analyses revealed that Bio-Normalizer inhibited OH radical, enhanced reduced GSH and stimulated SOD activities in either RBC or WBC and macrophage. These findings show that bio-Normalizer may be an important anti-inflammatory and anti-fibrotic agent.

2. Suppression by Antioxidative Chelators of Toxic Effects of Iron Overload (IOL) in Rats

Iron overload (IOL) is a well-known origin of human and animal pathologies initiated or mediated by free radicals. To investigate the possibility of the inhibition of iron-initiated damaging processes by antioxidative chelators, Bio-Normalizer researchers developed the animal models of IOL and studied the IOL effects on lipid peroxidation and oxygen radical production by neutrophils and macrophages. Iron overloading was achieved by feeding rat the diets supplemented with elemental iron for 42 days or by infusing the animals intraperitoneally with FeSO₄ solution for 3 days. To suppress iron-stimulated free radical processes, natural  nontoxic substances processing both chelating and antioxidant properties (bioflavonoid rutin, lipoic acid and Bio-Normalizer, a nutraceutical) to IOL rats. Rutin and lipoic acid diminished free radical overproduction without diminishing iron levels in the blood probably due to the formation of iron-rutin or iron-lipoic acid complexes, which were unable to catalyze free radical reactions. In contrast, Bio-Normalizer sharply decreased both the content of non-heme iron and lipid peroxidation in the plasmalemma of macrophages as well as the macrophage oxygen radical release. With the results obtained, it is assumed that toxic effects of iron overload may be suppressed  by antioxidative chelators via (1) scavenging free radicals, (2) the enhanced excretion of iron, (3) the formation of inactive iron-chelator complexes incapable of catalyzing free radical reaction.

Despite many studies in the field of biomedical sciences, the exact mechanisms underlying metal-associated cellular toxicity is,  in most cases not fully established yet. But the studies serve as stepping stone towards the discovery of safer and effective chelating and radical scavenging agents.